Various Topics on Aids

..t.. ..yn..

New England Cytometry User Group Home
(previously known as Boston Users Group for Cytometry ¨CBUG) ... C.
Bruce Bagwell, MD, Ph.D. - Verity Software House http://www.vsh.com/
...
www.bostoncytometry.org/NEChomepage.html - 16k - Cached - Similar
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MyCytometry
ISAC Purdue Cytometry List GLIIFCA. MyCyte.org is sponsored by Tree
Star, Inc. 340 A St. Ashland OR 97520 Phone: (541) 201-0022 Fax: (541)
482-3153.
www.flowcyte.com/ - 17k - Cached - Similar pages


ISAC Homepage - ISAC E-NEWS -- December 2004
j...@flowcyt.cyto.purdue.edu. Current Protocols in Cytometry (CPC) is
published in affiliation ... Royalties from sales of CPC come
directly
back to ISAC. ...
www.isac-net.org/content/view/249/119/ - 48k - Cached - Similar pages
- Note this


Molecular Expressions Microscopy Primer: University and
Government ...
Software is available to enable a variety of image enhancement
and ...
Purdue University Cytometry Laboratories (Purdue University) - This
website provides ...
micro.magnet.fsu.edu/primer/resources/facilities.html - 61k - Cached
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http://mycyte.org/content/view/112/108/
NIH is an Equal Opportunity Employer.
WHY IS THE PURDUE CYTOMETRY MAIL LIST LINK HERE TOO?

The University of Calgary Flow Cytometry Core Facility - Resources
Boston User's Group for Cytometry ¡¤ The Clinical Cytometry Society ...
Links ***ociated with flow cytometry research and related
information. ...
www.flowlab.ucalgary.ca/flowlab/resources.htm - 25k - Cached - Similar
pages

UM***/Amherst Flow Cytometry Facility
International Flow Cytometry Resources. Boston Flow Cytometry User's
Group BUG, a wealth of useful information, local, national and
international. ...
www.bio.um***.edu/mcbfacs/flowhome.html - 4k - Cached - Similar pages

Professional Organizations
BUG Boston Users Group http://www.bostoncytometry.org/. - Cascade
Cytometry User's Group Allen Kachelmeier at kachelme@ohsu.edu ...
http://www.flocyte.com/FRTP/Resource...anizations.htm - 23k -
Cached - Similar pages

Purdue Cytometry Mailing List: More BUG info
Hello, I have been asked to clarify that the fee for the Boston User
Group meeting on May 25 1995 includes dinner, drinks are extra.
Thanks. Peter Lopez ...
www.cyto.purdue.edu/hmarchiv/1995/0654.htm - 4k - Cached - Similar
pages


ISAC XXII International Congress
FlowJo software. Data showed optimal sensitivity was achieved using
PBMCs ...... CYTOMETRY: PURDUE UNIVERSITY. CYTOMETRY LABORATORIES. J.
Paul Robinson ...
doi.wiley.com/10.1002/cyto.a.20046.abs - Similar pages - Note this

Welcome to Flow Cytometry Core Facility
FlowJo Kinetics Tutorial. ¡ô FlowJo Software Reference Manuals: ...
Purdue flow cytometry. Technical Center for those using secondary and
tertiary ...
www.aecom.yu.edu/facs/ProtocolsLinks.html - 12k - Cached - Similar
pages - Note this

Flow Links - 9 visits - Mar 3
FlowJo Flojo help manual guide lesson help. ... J. Paul Robinson Gives
WikiPedia an "A" OK, the main Flow Cytometry entry got an A, but Dr.
Robinson felt ...
www.flowjo.com/home/links.html - 22k - Cached - Similar pages - Note
this

Fluorescence Compensation
Jennifer Wilshire, PhD jenni...@flowjo.com MetroFlow Meeting,
Princeton, NJ .... Purdue Cytometry Mailing list - http://www.cyto.purdue.edu.
Practical Flow ...
http://www.metroflow.org/archive/200...r_Wilshire.htm -
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http://www.healthsystem.virginia.edu...rms/FlowJo.cfm
FlowJo Site License
Registration Form
Complete the form below to access the University of Virginia's Site
License for FlowJo data analysis software. All fields with * must be
completed or your registration will not be processed. Once your
information has been received and verified you will receive a serial
number which you must enter in FlowJo on the computer whose hardware
address you provided. Cost per user license will be determined at the
end of the year depending on the average number of registered users
for the year. (See instructions for pricing structure) This amount
will be billed to the PTAO provided below, so please make sure the
PTAO you provide does not expire before that time. Completion of this
registration form is a valid request for services and ***umes the
approval of the Principal Investigator of the PTAO account provided.
Contact Joanne Lannigan joannelanni...@virginia.edu with questions.
Flow Links - 9 visits - Mar 3
FlowJo Flojo help manual guide lesson help. ... in Flow Cytometry -
Los Alamos. Purdue University Cytometry Laboratories * 2005 Mail List,
Thread Sort ...
www.flowjo.com/home/links.html - 22k - Cached - Similar pages - Note
this

Purdue Cytometry Mailing List: RE: Nonreadable FCS 3 files from
From : J. Paul Robinson <j...@flowcyt.cyto.purdue.edu> ... We are
currently working on adding the support of these files in the Windows
> version of FlowJo, ...

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Purdue Cytometry Mailing List: Re: Nonreadable FCS 3 files from
From : J. Paul Robinson <j...@flowcyt.cyto.purdue.edu> ... For data
analysis we want to > use a centraly designed templates made in Flow
Jo software. ...
www.cyto.purdue.edu/hmarchiv/2004/0997.htm - 8k - Cached - Similar
pages - Note this

The Science Advisory Board - Protocols, Product Reviews, Member ... -
48 visits - Mar 3
Flow Cytometry: A Brief Introduction and Guide to On-Line
Resources ... Dr. J. Paul Robinson, Purdue University School of
Veterinary Medicine, ...
http://www.scienceboard.net/communit...tives.169.html - 30k - Cached -
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Useful Links & Resources - Flow Cytometry Site
General. Purdue University Cytometry Labs (J. Paul Robinson) ...
Software. FlowJo. http://flowjo.com/. FACSPress and FACS***istant.
http://www.fcspress.com/ ...
idi.harvard.edu/content/facilities/cytometry/website/pages/links.html
- 42k - Cached - Similar pages - Note this

Meeting 2007
J Paul Robinson (Purdue University, USA). Multicolour Flow
Cytometry ... Cytek Development, Distrilab, FlowJo, Guava
Technologies, Invitrogen, ...
www.flowcytometryuk.org/fliers/Annual1.html - 8k - Cached - Similar
pages - Note this

Unitat de Citometria - Llibreria
J.Paul Robinson ISBN 0-471-59634-5 Wiley-Liss 1993 Flow Cytometry in
Clinical Diagnosis ... Blog dedicat al programa FlowJo; Maeckerlab
Weblog. ...
citometria.germanstrias.org/cat/llibreria.htm - 13k - Cached - Similar
pages - Note this

[PDF]
Tree Star
File Format: PDF/Adobe Acrobat - View as HTML
We report the use of flow cytometry to measure fluorescence ...... J.
Paul Robinson. j...@flowcyt.cyto.purdue.edu. Philip Marder.
phil.mar...@lilly.com ...
www.gliifca.org/pdf/GLIIFCA-2004.pdf - Similar pages - Note this

[PDF]
GLIIFCA15
File Format: PDF/Adobe Acrobat - View as HTML
Todd Lary (4), and J. Paul Robinson (1). (1) Purdue University
Cytometry Laboratories, ..... Acquired data was subsequently analyzed
using FlowJo (Treestar, ...
www.gliifca.org/pdf/GLIIFCA15Program.pdf - Similar pages - Note this

PPT]
Hons lecture 2005
File Format: Microsoft Powerpoint - View as HTML
2002 J.Paul Robinson, Purdue University. Stokes Shift .... Flowjo.
WinMDI. Analysis software. Flow Cytometry on the web e.g., ...
web.uct.ac.za/depts/biomed/burger.PPT - Similar pages - Note this

[PDF]
THURSDAY, MARCH 16, 2006
File Format: PDF/Adobe Acrobat - View as HTML
New Applications in Particle Analysis Using Concurrent Flow Cytometry
and ..... JO, University of Memphis, Gija Geme, Lucy J Thurston,
Angela L Howard, ...
http://www.pittcon.org/archive/2006/...0AM%20Program%...
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Unidad de Citometr¨ªa - Librer¨ªa
- [ Translate this page ]
Blog dedicado al programa FlowJo; Maeckerlab Weblog. ... J.Paul
Robinson ISBN 0-471-59634-5 Wiley-Liss 1993 Flow Cytometry in Clinical
Diagnosis ...
citometria.germanstrias.org/cast/libreria.htm - 12k - Cached - Similar
pages - Note this

Purdue Cytometry Mailing List: FlowJo and CellQuest (was RE: OS9
I touch the flow cytometer while it collects all my data AND I always
use FlowJo for my analysis. No, FlowJo is not a solution for
acquisiton but again I ...
www.cyto.purdue.edu/hmarchiv/2000/0775.htm - 9k - Cached - Similar
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Purdue Cytometry Mailing List: Flow Jo for PC
Dear All Am I correct in thinking that there is a PC version of Flow
Jo coming out soon? Best wishes Simon Watson Phagocyte Lab MRC Centre
for Inflammation ...
www.cyto.purdue.edu/hmarchiv/2002/0944.htm - 5k - Cached - Similar
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Purdue Cytometry Mail List ISAC FlowJo I hope you will excuse a ...
Message from discussion Purdue Cytometry Mail List ISAC FlowJo I hope
you will excuse a Bunch of blatantly Commercial Annoucements to the
list, but Indulge ...

Re: FlowJo
From: Carmen Raventos-Suarez (carmen.raventossua...@bms.com)
Date: Thu Jun 21 2001 - 16:49:26 EST
* Next message: Phil Marder: "Re: FlowJo"
* Previous message: Emmanuel Gustin: "Re: power meter"
* In reply to: Manfra, Denise: "FlowJo"
* Next in thread: Phil Marder: "Re: FlowJo"
* Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
Denise and everybody else interested in Flow Jo:
I have been using Flow Jo for a year and my requirements are very
much
like
yours. I also do a lot of DNA analysis, apoptosis and proliferation
***ays.
We just use the Fl-1, Fl-2, etc. as defaults. That way we can run
different
set of samples using several different markers with the same dye at a
single
run. Later in the analysis we add to the plots the specific name of
the
markers. It is fast and efficient, I definitely love it, my analysis
time has
been cut down in an exponential way. You create a template and can
drop buckets
of samples in it to be analyzed in no time. This includes time for
you
to view
every single plot in a movie sequence to be sure that all your gates
are
correct. Still if you have cells from different origins and need to
correct
gates, this can be done for the individual samples that require
correction
leaving the rest untouchable. And the results on excel are at your
finger tips
customized by your needs in a couple of c****s. I will never forget
all the
macros I needed to built to create individual excel tables from cell
quest.
I fewer words, as I told BD who where the ones who introduce me to
Flow Jo:
"Flow Jo is like to live in the future and there is no way you want
to
return
to the past".
Carmen

- Hide quoted text -
- Show quoted text -
"Manfra, Denise" wrote:
> Hi:
> I wanted some feedback on the FLOW Jo application. I
> currently do all of my analysis with CellQuest. I am wondering if FLOW Jo
> may expedite my analysis. However, I do not use the same markers all the
> time, and in one experiment I may be running more than one stain with
> multiple different antibodies. Basically, very seldom do I run the same set
> of markers. How does FLOW Jo work for such conditions?? How about plot
> analysis and excel: apparently Excell FLOW Jo puts the data directly into
> EXcell?? What has been your experience with FLOW Jo?? Any information would
> be useful.
> denise
> ************************************************** *************
> This message and any attachments is solely for the intended recipient. If
> you are not the intended recipient, disclosure, copying, use, or
> distribution of the information included in this message is prohibited --
> please immediately and permanently delete this message.

* text/x-vcard attachment: Card for Carmen Raventos-Suarez
* Next message: Phil Marder: "Re: FlowJo"
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* In reply to: Manfra, Denise: "FlowJo"
* Next in thread: Phil Marder: "Re: FlowJo"
* Messages sorted by: [ date ] [ thread ] [ subject ] [ author ]
[ attachment ]
This archive was generated by hypermail 2.1.6 : Thu Jan 01 2004 -
17:40:22 EST
FACS Flow Cytometry Facility
Cytometry Users E-mail archive (easily searchable):
http://www.cyto.purdue.edu/hmarchiv/cytomail.htm. Flow cytometry
analysis software: FACS Core Facility ...
www.immunology.utoronto.ca/CellSorter.htm - 24k - Cached - Similar
pages - Note this

FACS Flow Cytometry Facility
Cytometry Users E-mail archive (easily searchable):
http://www.cyto.purdue.edu/hmarchiv/cytomail.htm. Flow cytometry
analysis software: FACS Core Facility ...
www.immunology.utoronto.ca/CellSorter.htm - 24k - Cached - Similar
pages - Note this

The Daily Dongle: Science
Rant about FlowJo's goings-on. ... This post from the Cytometry
Mailing list answers a question I got earlier today:. Scale (linear)
Value = f * 10^ [(C ...
flowjo.typepad.com/the_daily_dongle/science/index.html - 27k - Cached
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The Daily Dongle:
I saw this question on Purdue mailing list earlier today. Since FlowJo
supports both methods, it's probably worth commenting on: ...
flowjo.typepad.com/the_daily_dongle/2007/06/index.html - 33k - Cached
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Purdue Cytometry Mailing List: FACS sample files
... gmail.com> Date: Tue Apr 10 2007 - 07:31:56 EDT ... like to try
the Winlist software to plan whether or not this software would fit
to
our routine ...
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Purdue Cytometry Mailing List: Re: Measuring FL-A and FL-W
... Purdue Cytometry Mailing List: Re: Measuring FL-A and FL-W ... in
your FACScan? I've never tried, but I ***ume you can tell the
software
the machine has DDM when in fact it does not. That would explain
your ...
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Purdue Cytometry Mailing List: By Date
... Wed Dec 07 2005 - 14:07:26 EST ... WinMDI software - long
filename
problem WAS Doubts About WinMDI software Alan Bishop ...
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Purdue Cytometry Mailing List: By Subject
... Purdue Cytometry Mailing List: By Subject ... Ray Hester (Thu Oct
07 2004 - 16:17:26 EST) ...
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Purdue Cytometry Mailing List: By Author
... Purdue Cytometry Mailing List: By Author ... WinMDI software -
long filename problem WAS Doubts About WinMDI software (Thu Dec 01
2005 - 19:07:54 EST) ...
http://www.cyto.purdue.edu/hmarchiv/2005/author.htm - 319.5KB
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Purdue Cytometry Mailing List: By Subject
... Now with website) My Flow Cytometry software website ...
vecoe...@usp.br (Tue Jul 19 2005 - 16:17:28 EST) ... FACSDiva re-
installation? ... Dr Natalie McCloskey (Thu Nov 10 2005 - 07:25:07
EST) ...
http://www.cyto.purdue.edu/hmarchiv/2005/subject.htm - 311.2KB
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Purdue Cytometry Mailing List: Applications Specialist - iCyt -
... Purdue Cytometry Mailing List: Applications Specialist - iCyt ...
Abdul-Jabbar [mailto:iajab...@cicr.uq.edu.au] Sent: Wednesday,
February 14, 2007 8:41 PM To: cyto-inbox Subject: DNA analysis
software ...
http://flowcyt.cyto.purdue.edu/hmarc...rrent/0237.htm - 6.6KB
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Purdue Cytometry Mailing List: RE: DiVa software on Intel Mac pr
... Purdue Cytometry Mailing List: RE: DiVa software on Intel ...
Richard D. Schretzenmair [mailto:r...@mail.med.upenn.edu] Sent:
Wednesday, January 03, 2007 9:09 AM To: cyto-inbox ...
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Purdue Cytometry Mailing List: DiVa software on Intel Mac proble
... Purdue Cytometry Mailing List: DiVa software on Intel Mac ...
From: Richard D. Schretzenmair <r...@mail.MED.UPENN.EDU> Date: Wed
Jan
03 2007 - 11:09:03 EST ...
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Purdue Cytometry Mailing List: RE: Diva 5.0 Software on Aria
... Purdue Cytometry Mailing List: RE: Diva 5.0 Software on Aria ...
Contemporary messages sorted: [ By Date ] [ By Thread ] [ By
Subject ]
[ By Author ] [ By messages with attachments ...
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Purdue Cytometry Mailing List: New England Cytometry Users Group
... Purdue Cytometry Mailing List: New England Cytometry Users
Group ... Spherotech Stem Cell Technologies Union Biometrica
Upstate /
Millipore ViaCell Verity Software House And I would like to thank my
colleagues ...
http://www.cyto.purdue.edu/hmarchiv/2006/1625.htm - 6.4KB
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Purdue Cytometry Mailing List: SV: Thanks for the suggestions -
... cytometry/WEASELv2.html> http://www.wehi.edu.au/cytometry/WEASELv2.html
Winlist 3d from Verity House Software (www.vsh.com) Regards Tim
Timothy Bushnell, Ph.D. Director, CPBR ... Contemporary messages
sorted: [ By Date ] [ By Thread ] [ By Subject ] [ By Author ...
http://www.cyto.purdue.edu/hmarchiv/2006/0914.htm - 7.0KB
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Purdue Cytometry Mailing List: Thanks for the suggestions - was
... the FC500 Rflowcyt Weasel: http://www.wehi.edu.au/cytometry/WEASELv2.html
Winlist 3d from Verity House Software (www.vsh.com) ... edu.au/
cytometry/WEASELv2.html Winlist 3d from Verity House Software
(www.vsh.com) ...
http://www.cyto.purdue.edu/hmarchiv/2006/0902.htm - 6.6KB
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Purdue Cytometry Mailing List: 29th ANNUAL COURSE IN FLOW CYTOME
... Annual Course in Cytometry Organizer: C. Bruce Bagwell, MD, Ph.D.
Verity Software House, Inc. PO Box 247 Topsham, ME ... Organizer: C.
Bruce Bagwell, MD, Ph.D. Verity Software House, Inc. PO Box 247
Topsham, ME 04086 ...
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Purdue Cytometry Mailing List: 29th ANNUAL COURSE IN FLOW CYTOME
... Purdue Cytometry Mailing List: 29th ANNUAL COURSE IN FLOW
CYTOME ... in Cytometry Organizer: ... C. Bruce Bagwell, MD, Ph.D.
Verity Software House, Inc. PO Box 247 Topsham, ME 04086 Tel: (207)
729-6767 x102 Email ...
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Purdue Cytometry Mailing List: 2006 ANNUAL COURSE IN FLOW CYTOME
... Annual Course in Cytometry Organizer: C. Bruce Bagwell, MD, Ph.D.
Verity Software House, Inc. PO Box 247 Topsham, ME ... Organizer: C.
Bruce Bagwell, MD, Ph.D. Verity Software House, Inc. PO Box 247
Topsham, ME 04086 ...
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Purdue Cytometry Mailing List: RE: Boston Users Group for Cytome
... Purdue Cytometry Mailing List: RE: Boston Users Group for
Cytome ... Gene Check Applied Technologies Evotec Technologies Guava
Technologies iCyt Invitrogen / Molecular Probes Verity Software
House ...
http://www.cyto.purdue.edu/hmarchiv/2005/1602.htm - 8.0KB
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Purdue Cytometry Mailing List: RE: ModFit LT on G4
... Purdue Cytometry Mailing List: RE: ModFit LT on G4 ... regards,
Don Donald J. Herbert Technical Support Manager Verity Software House
PO Box 247 45A Augusta Road Topsham, Maine 04086 t...@vsh.com www.vsh
...
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Purdue Cytometry Mailing List: RE: DNA PLOIDY AND S-PHASE
... Spyrantos, DrSc; et al: in Cytometry (Communications in Clinical
Cytometry) 46:121-135 (2001) Published ... J. Herbert Technical
Support Manager Verity Software House, Inc. PO Box 247 ...
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Purdue Cytometry Mailing List: RE: FACS simulator?
... Purdue Cytometry Mailing List: RE: FACS simulator? ... Donald J.
Herbert Technical Support Manager Verity Software House, Inc. PO Box
247 45A Augusta Road Topsham, ME, USA 04086 ...
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Purdue Cytometry Mailing List: FACScan for Sale
... Purdue Cytometry Mailing List: FACScan for Sale ... sale. ... The
Scan has been interfaced with a Cicero Box with Cyclops Summit
software. It has been serviced regularly since purchased in 1996. The
laser and power ...
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Purdue Cytometry Mailing List: Annual Research Course in Flow Cy
... The Los Alamos National Flow Cytometry Resource (NFCR), And
Verity
Software House ... Contemporary messages sorted: [ By Date ] [ By
Thread ] [ By Subject ] [ By Author ] [ By messages with
attachments ...
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Purdue Cytometry Mailing List: The Clinical Cytometry Society Cy
... Purdue Cytometry Mailing List: The Clinical Cytometry Society
Cy ... Verity Software House is proud to sponsor the Clinical
Cytometry Society Cyber Caf¨¦ ...
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Purdue Cytometry Mailing List: Heads up for people using WinZip
... Purdue Cytometry Mailing List: Heads up for people using
WinZip ... Donald J. Herbert Technical Support Manager Verity
Software
House, Inc. PO Box 247 45A Augusta Road Topsham, ME, USA 04086
Phone ...
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Purdue Cytometry Mailing List: CD8 available upon request
... Purdue Cytometry Mailing List: CD8 available upon request ...
Cytomation, Molecular Probes, Phoenix Flow Systems, Trillium
Diagnostics, Verity Software, Chroma Filters, Evergreen Laser, Tree
Star, Southern Biotechnology ...
http://www.cyto.purdue.edu/hmarchiv/2004/1178.htm - 6.5KB
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Purdue Cytometry Mailing List: Re: FC500 files - parameter name
... read them from 3rd party software programs....so if you use the
fc500 listmode software to generate 2P histograms that ... analysis >
with WinList and notified Verity; they have added an option ...
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Purdue Cytometry Mailing List: Coulter Altra For Sale
... Purdue Cytometry Mailing List: Coulter Altra For Sale ...
Manufactured in October 1998 and running Expo 32 Software. Serial
Number: AB32006 Under Service Contract until 2002. ...
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Purdue Cytometry Mailing List: Pre-GLIIFCA DNA course
... Purdue Cytometry Mailing List: Pre-GLIIFCA DNA course ... Hi
Flowers, > FloCyte ***ociates and Verity Software announce a PRE-
GLIIFCA > course in DNA analysis. Want to get the best out of
your ...
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Purdue Cytometry Mailing List: Pre-GLIIFCA DNA course Deadline...
... Purdue Cytometry Mailing List: Pre-GLIIFCA DNA course
Deadline... ... Hi Flowers, > FloCyte ***ociates and Verity Software
announce a PRE-GLIIFCA > course in DNA analysis. Want to get the best
out ...
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http://flowjo.typepad.com/the_daily_.../06/index.html

June 29, 2007
NIH Funding

Another thread from Purdue worth posting:

We are calling upon you again during this Independence Day
holiday
period to ask you to contact your Representative in person at a local
event or by telephone to the local office by July 9th. When Congress
returns to Washington after the 4th of July recess, the House of
Representatives will consider a 1.9% increase to the NIH budget.
Between now and then, you have the opportunity to remind your
Representative of the importance of your work and let them know about
any difficulties you have encountered because of shrinking budgets.

Members of Congress have told us that they are not hearing from
researchers in the community. We must turn this around. Members and
their staffs are receptive to hearing from researchers about how the
proposed FY 2008 budget will affect their research and their careers.

Please contact your Representative either in person at a local
Independence Day event or by telephone before July 9th. Tell them
your
story and let them know that they must make cancer and biomedical
research funding a national priority.

In your contact with your Representative, please mention any of
the research challenges you are facing at this time, for example: -
denials or delays in getting grant funding for meritorious research
-the impact of cuts to grants already funded
-staffing and equipment shortages
-the loss of bright young investigators to other fields
-reduced opportunities for patients to get on therapeutic
clinical
trials
-cancellation or halting of important clinical trials.

In short, your Representative needs to hear about the serious
effect these low budgets are having on your research progress. Urge
them to make cancer research funding a national priority. A few
minutes of your time could help get NIH and NCI funding back on
track.

Thank you for responding to this request and have an enjoyable
holiday.

William N. Hait, M.D., Ph.D., President
William G. Nelson V, M.D., Ph.D., Chair, Science Policy &
Legislative Affairs Committee
Margaret Foti, Ph.D., M.D. (h.c.), Chief Executive Officer

P.S. Please let AACR know you have made a contact with your
Representative by sending us a quick e-mail at legaffa...@aacr.org.
Just write: "I have contacted Representative X in person/by phone."

June 29, 2007 in Current Affairs | Permalink | Comments (0)
June 27, 2007
http://flowjo.typepad.com/the_daily_.../06/index.html
************************************************** *************************-
*********************************************
Re: mr on Apple web site
* This message: [ Message body ] [ More options ]
* Related messages: [ Next message ] [ Previous message ] [ In
reply
to ] [ Next in thread ] [ Replies ]
From: Howard Shapiro <h...@shapirolab.com>
Date: Wed Aug 25 2004 - 18:15:38 EST

Robin Barclay wrote:
>Well .... some of us PC afficianados hate Macs just as much - personally I
>have hated them since they first made it difficult to access their DOS and
>write programs in any language (especially for accessing/controlling lab
>apparatus) - back in the '70's when there were several different options -
>not just PC's. I am glad that the "PC" became a standard (there were too
>many diferent systems) and that IBM did not hang on to it the way Apple kept
>the Mac in house (you get more for your money with a PC because many
>different people make them). You don't often get PC people knocking Macs
>the way that the Mac people knock PCs - there seems to be a lot of Microsoft
>paranoia. In my opinion PCs are much more common and versatile in labs than
>Macs - especially outside the USA - and will eventually become the standard
>for interfacing with lab equipment..... and they can "look cool" if you shop
>around for the right case if that's important to you.

I can't p*** up the opportunity to get into the PC/Mac battle - I
hate
them
both, but there aren't really alternatives (yes, I know, there's
Linux, but
it's probably even harder to hook up a Linux system to hardware than
it is
to hook a Mac up to hardware).

Of course, what started this thread was the ***ertion in the piece
about
Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
flow
cytometers out there. The best estimates I had from industry people
in
early 2003 when I was finishing up the 4th Edition of PFC was that
the
total number of systems from all manufacturers in use was under
20,000. If
that's correct, BD would need a 75% market share to account for the
low end
estimate of 15,000 Mac-based machines. I'd be interested to know
where
the
15,000 to 20,000 figure came from.

The old (68000 series and possibly early PowerPC) Macs were
difficult,
but
not impossible to connect to hardware; it was easier to work with the
PC's
ISA bus, which, while slow, was perfectly adequate to do most flow
cytometric data acquisition and analysis. There were decent versions
of
Forth, which was one of the first and best languages designed for
controlling hardware from mini- and microcomputers, for both PC (DOS)
and
the Mac (Forth was the first Mac programming language made
commercially
available, at a time when the only other option was buying the very
expensive Lisa from Apple on which to develop Mac software). I found
MacForth easier to program with than the Windows Forths (or other
Windows
languages, e.g., Delphi), but my old Macs used to crash all the time.

So what makes me unhappy with both Windows [and Windows machines] and
the
Mac in their current incarnations? It is now significantly harder for
mere
mortals to write software to get hardware to communicate with either
PCs or
Macs; the gain in complexity ***ociated with the PCI bus, USB/USB2,
FireWire(IEEE 1394), etc. is greater than the gain in speed and
convenience. Also, in making the operating systems more stable (and
Win XP,
despite its security issues, is almost as stable as Mac's Unix-based
OS X),
both Microsoft and Apple elected to eliminate the ability of their
computer
hardware to respond rapidly to interrupts (latencies are now in the
tens of
thousands of instruction cycles), meaning that any really fast
hardware
attachment for either a PC or a Mac now needs to have a DSP in it,
whereas
if the fast interrupt response had been preserved, the hardware
attachments
could have been made much simpler and cheaper. Linux also takes fast
interrupt response off the table, so it doesn't represent a viable
alternative. If you go to Apple's web site and look at what data
acquisition hardware is available for Macs, particularly for Macs
running
OS X, there isn't much, and many of the companies that supported the
Mac in
that area have dropped Mac support for their newer products. That is
undoubtedly one big reason why BD's newer digital pulse processing
cytometers are running on the PC platform.

That doesn't stop anybody from analyzing FCS files on Macs. FlowJo is
well-conceived flow cytometry software; one reason it is as good as
it
is
is that it was written by people who do a lot of flow cytometry, and
cutting edge flow cytometry at that. But there are other people who
do
a
lot of good flow cytometry who have written good software, for PCs as
well
as for Macs.

For the record, I have a G4 PowerBook, which I use mostly for the
iLife
applications, which are s****. It doesn't crash more than once every
couple
of months, but the same is true of my Windows 2000 and XP systems. OS
X can
be as infuriating as Windows when one or another aspect of it goes
counter
to your intuition or to what you have gotten used to. Macs, while
somewhat
more expensive, are much better made than many PCs, and they are
certainly
aesthetically pleasing. If there were a reasonable alternative to
Microsoft's Office applications for the Mac, I might consider
switching.
The 12.1" PowerBook is a nice portable, but it's over a pound heavier
than
the Fujitsu laptop I now use, which has pretty much the same speed,
memory,
and drive capabilities (OK, not DVD-R, but I don't burn a lot of
DVDs).

However, I really wish Apple had stuck with the plan they had a few
years
back of writing a Mac operating system for Intel hardware. I think
that
died when Microsoft bailed Apple out with a few hundred mil. If it
were
possible to run OS X and XP mano a mano on the same hardware, there'd
be a
more rational basis for comparison. But, as may be the case for the
November election, minds, once made up, are not easily changed.

-Howard
Received on Thu Aug 26 16:38:00 2004
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Re: mr on Apple web site
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hmarchiv/
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From: Howard Shapiro <h...@shapirolab.com
<mailto:h...@shapirolab.com?
subject=Re:%20mr%20on%20Apple%20web%20site>>
Date: Wed Aug 25 2004 - 18:15:38 EST

Robin Barclay wrote:
>Well .... some of us PC afficianados hate Macs just as much - personally I
>have hated them since they first made it difficult to access their DOS and
>write programs in any language (especially for accessing/controlling lab
>apparatus) - back in the '70's when there were several different options -
>not just PC's. I am glad that the "PC" became a standard (there were too
>many diferent systems) and that IBM did not hang on to it the way Apple kept
>the Mac in house (you get more for your money with a PC because many
>different people make them). You don't often get PC people knocking Macs
>the way that the Mac people knock PCs - there seems to be a lot of Microsoft
>paranoia. In my opinion PCs are much more common and versatile in labs than
>Macs - especially outside the USA - and will eventually become the standard
>for interfacing with lab equipment..... and they can "look cool" if you shop
>around for the right case if that's important to you.

I can't p*** up the opportunity to get into the PC/Mac battle - I
hate
them
both, but there aren't really alternatives (yes, I know, there's
Linux, but
it's probably even harder to hook up a Linux system to hardware than
it is
to hook a Mac up to hardware).

Of course, what started this thread was the ***ertion in the piece
about
Mario on Apple's web site that there were 15,000 to 20,000 Mac-based
flow
cytometers out there. The best estimates I had from industry people
in
early 2003 when I was finishing up the 4th Edition of PFC was that
the
total number of systems from all manufacturers in use was under
20,000. If
that's correct, BD would need a 75% market share to account for the
low end
estimate of 15,000 Mac-based machines. I'd be interested to know
where
the
15,000 to 20,000 figure came from.

The old (68000 series and possibly early PowerPC) Macs were
difficult,
but
not impossible to connect to hardware; it was easier to work with the
PC's
ISA bus, which, while slow, was perfectly adequate to do most flow
cytometric data acquisition and analysis. There were decent versions
of
Forth, which was one of the first and best languages designed for
controlling hardware from mini- and microcomputers, for both PC (DOS)
and
the Mac (Forth was the first Mac programming language made
commercially
available, at a time when the only other option was buying the very
expensive Lisa from Apple on which to develop Mac software). I found
MacForth easier to program with than the Windows Forths (or other
Windows
languages, e.g., Delphi), but my old Macs used to crash all the time.

So what makes me unhappy with both Windows [and Windows machines] and
the
Mac in their current incarnations? It is now significantly harder for
mere
mortals to write software to get hardware to communicate with either
PCs or
Macs; the gain in complexity ***ociated with the PCI bus, USB/USB2,
FireWire(IEEE 1394), etc. is greater than the gain in speed and
convenience. Also, in making the operating systems more stable (and
Win XP,
despite its security issues, is almost as stable as Mac's Unix-based
OS X),
both Microsoft and Apple elected to eliminate the ability of their
computer
hardware to respond rapidly to interrupts (latencies are now in the
tens of
thousands of instruction cycles), meaning that any really fast
hardware
attachment for either a PC or a Mac now needs to have a DSP in it,
whereas
if the fast interrupt response had been preserved, the hardware
attachments
could have been made much simpler and cheaper. Linux also takes fast
interrupt response off the table, so it doesn't represent a viable
alternative. If you go to Apple's web site and look at what data
acquisition hardware is available for Macs, particularly for Macs
running
OS X, there isn't much, and many of the companies that supported the
Mac in
that area have dropped Mac support for their newer products. That is
undoubtedly one big reason why BD's newer digital pulse processing
cytometers are running on the PC platform.

That doesn't stop anybody from analyzing FCS files on Macs. FlowJo is
well-conceived flow cytometry software; one reason it is as good as
it
is
is that it was written by people who do a lot of flow cytometry, and
cutting edge flow cytometry at that. But there are other people who
do
a
lot of good flow cytometry who have written good software, for PCs as
well
as for Macs.

For the record, I have a G4 PowerBook, which I use mostly for the
iLife
applications, which are s****. It doesn't crash more than once every
couple
of months, but the same is true of my Windows 2000 and XP systems. OS
X can
be as infuriating as Windows when one or another aspect of it goes
counter
to your intuition or to what you have gotten used to. Macs, while
somewhat
more expensive, are much better made than many PCs, and they are
certainly
aesthetically pleasing. If there were a reasonable alternative to
Microsoft's Office applications for the Mac, I might consider
switching.
The 12.1" PowerBook is a nice portable, but it's over a pound heavier
than
the Fujitsu laptop I now use, which has pretty much the same speed,
memory,
and drive capabilities (OK, not DVD-R, but I don't burn a lot of
DVDs).

However, I really wish Apple had stuck with the plan they had a few
years
back of writing a Mac operating system for Intel hardware. I think
that
died when Microsoft bailed Apple out with a few hundred mil. If it
were
possible to run OS X and XP mano a mano on the same hardware, there'd
be a
more rational basis for comparison. But, as may be the case for the
November election, minds, once made up, are not easily changed.

-Howard
Received on Thu Aug 26 16:38:00 2004
* This message: [ Message body <http://www.cyto.purdue.edu/
hmarchiv/
2004/1485.htm> ]
* Next message: Konz, Richard: "Discrimination of GO/G1"
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RE: mr on Apple web site
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[ Maybe in
reply to ]
From: Fischer, Randy (NIH/NIAMS) <fisch...@mail.nih.gov>
Date: Mon Aug 23 2004 - 16:18:55 EST
Paul,

I know that for a long time, anyone who used a BD flow cytometer had
little
choice but to use a MAC-data acquisition required it. And, one of
the
best
third party software packages was FlowJo which only ran on a MAC.
Mario,
being a FlowJock, naturally was heavily exposed to this atmosphere.
However, most of our other laboratory instrumentation appears to run
on the
IBM clone type of PC-ELISA reader, luminometer, ELISpot reader, and
AFFYMETRIX machine to name the first ones that come to mind. Of
course,
because Flow is such a key component and FlowJo works so well, we
have
MACs
(and ***ociated cinema displays) at all of our desks. So, Mario is
probably
pretty close as I guess we now run about 50% MAC, and for most things
they
do run better than our PCs for similar applications, but mostly the
two
platforms tend to be used for very disparate applications and we need
both.

Wish more science companies would move to MAC, but as Microsoft
probably
owns a minority share in most companies, that will not likely happen
in our
lifetimes.

Haven't read the article yet, but hopefully Mario remembered to cite
you for
all the work on the Flow website/mailing list.

Randy T. Fischer
NIH/NIAMS
Building 10, Room 6D50
9000 Rockville Pike
Bethesda, MD 20892
(301) 594-3537
fisch...@mail.nih.gov

ISAC Purdue Cytometry List GLIIFCA. MyCyte.org is sponsored by Tree
Star, Inc.
http://mycyte.org/content/view/93/103/

PURDUE CYTOMETRY MAIL LIST

I have some strong words to end the year and I am going to say them!!
Of course
you don t have to read them!

Cytometry is now 40 years old and it s been sort of decaying a bit.
What
do I mean? I am amazed at how conservative and frankly boring the
field
has become. Why?

Wake up people - times are changing - look at all these new small
companies trying to stick their noses in "our" field!

Wake up people - times are changing - look at all these new small
companies trying to stick their noses in "our" field!


http://groups.google.com/group/misc....f654cee64a8cf1